ALI Component	Concentration
LHC Basal Medium	50% of medium
DMEM high glucose with pyruvate	50% of medium
Glutamine	2mM
Bovine serum albumin	0.5mg/ml
Bovine pituitary extract	0.24mg/ml
Insulin	5μg/ml
Transferrin	10μg/ml
Hydrocortisone	0.1μM
Triiodothyronine	0.01μM
Epinephrine	2.7μM
Recombinant human EGF	0.5ng/ml
Retinoic acid	0.05μM
Phosphorylethanolamine	0.5μM
Ethanolamine	0.5μM
Zinc sulfate	3μM
Penicillin, Streptomycin	100U/ml, 100μg/ml
Selenium	0.03μM
Manganese	0.001μM
Silicone	0.5μM
Molybdenum	0.001μM
Vanadium	0.005μM
Nickel sulfate	0.001μM
Tin	0.0005μM
Ferrous sulfate	1.5μM
Magnesium chloride	0.6mM
Calcium chloride	0.11mM