KEY RESOURCES TABLE
| REAGENT or RESOURCESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| Heat-Killed P. aeruginosa laboratory strain PAO1 | InvivoGen | Cat#tlrl-hkpa |
| Mucoid P. aeruginosa clinical strain | UCSF Microbiology | Acc#F24698 |
| P. aeruginosa clinical strain | UCSF Microbiology | Acc#S59951 |
| Biological Samples | ||
| Airway explant tissue | UCSF Department of Pathology | IRB 10-02253 |
| Airway brush samples | UCSF Dep. of Medicine | IRB 13-10738 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Rho kinase inhibitor, Y-27632 | Selleck Chem | Cat# S1049 |
| QIAzol | QIAGEN | Cat#79306 |
| TRIzol | Invitrogen | Cat#15596026 |
| Trypsin-EDTA 0.25% | ThermoFisher | Cat#25200056 |
| Protease from Streptomyces griseus Type XIV | Millipore Sigma | Cat#P5147 |
| Tryptic Soy Broth | BD-Bacto | Cat#211825 |
| Critical Commercial Assays | ||
| miRNeasy Mini Kit | QIAGEN | Cat#217004 |
| SuperScript cDNA Synthesis Kit | Invitrogen | Cat#11904018 |
| Taqman Gene Expression Master Mix | Applied Biosystems | Cat#4369542 |
| Human Cytokine/Chemokine Array 65-Plex Panel | Eve Technologies | Cat#HD65 |
| NEBNext RNA First Strand Synthesis Module | New England BioLabs | Cat#E7525 |
| NEBNext Ultra® Second Strand Synthesis Module | New England BioLabs | Cat#E7550 |
| NEBNext Ultra® II DNA Library Prep Kit for Illumina | New England BioLabs | Cat#E7645 |
| GenElute Mammalian Genomic DNA Miniprep Kit | Millipore Sigma | Cat#G1N70 |
| QIAshredder | QIAGEN | Cat#217084 |
| Human Cytokine/Chemokine Array | Eve Technologies | Cat#HD71 |
| Human Adipokine Array 5-Plex | Eve Technologies | Cat#HDADK5 |
| Infinium MethylationEpic Array | Illumina | Cat#WG-317-1002 |
| Deposited Data | ||
| Airway Epithelial Transcriptome in Lung Transplant Recipients with Cystic Fibrosis and/or Pseudomonas | Mendeley Data | https://doi.org/10.17632/89nzfyvkvc.1 |
| Experimental Models: Cell Lines | ||
| Primary cell cultures derived from explanted tissue | See Biological Samples above | n/a |
| Experimental Models: Organisms/Strains | ||
| Pseudomonas aeruginosa | See Bacterial and Virus Strains above | n/a |
| Oligonucleotides | ||
| Cxcl8 Hs00174103_m1 | ThermoFisher | Cat#4331182 |
| Lcn2 Hs01008571_m1 | ThermoFisher | Cat#4331182 |
| Mx1 Hs00895608_m1 | ThermoFisher | Cat#4331182 |
| Oas1 Hs00973637_m1 | ThermoFisher | Cat#4331182 |
| Actb Hs01060665_g1 | ThermoFisher | Cat#4331182 |
| Stat1 Hs01013996_m1 | ThermoFisher | Cat#4331182 |
| Other | ||
| ALI Transwell® Inserts | Corning Life Sciences | Cat#3460 |