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. Author manuscript; available in PMC: 2020 Sep 25.
Published in final edited form as: Eur J Immunol. 2020 May 28;50(9):1386–1399. doi: 10.1002/eji.201948455

Figure 7.

Figure 7.

PI3Kδ reduces differentiation of human Meso-CAR T cells while preserving cytolytic capacity. Healthy donor T cells were stimulated with anti-CD3/CD28 beads and transduced with a mesothelin-specific 4–1BBζ CAR in the presence or absence of PI3K inhibitors. (A) Expansion of vehicle and inhibitor-treated CAR T cell cultures over 2 weeks. (B) Representative flow cytometry plots of CD4+ CAR+ (top) and CD8+ CAR+ (bottom) T cell memory profiles on Day 7 of expansion. Expression of costimulatory and inhibitory receptors of CD4+ CAR+ (C) and CD8+ CAR+ (D) T cells on Day 7 of expansion. (E) Representative flow cytometry plots and quantification of K562-meso cell death with total T cells in inhibitor-free media on Day 15. Cell growth analyzed by one-way ANOVA with Tukey’s multiple comparisons at each time point. Surface expression and cytotoxicity data analyzed by one-way ANOVA with Tukey’s multiple comparisons, n = 6 healthy donors/group from two independent experiments. All bars represent the mean ± the SD with statistical significance as p < 0.05*, p < 0.01**, p < 0.001***, and p < 0.0001****.