Skip to main content
. Author manuscript; available in PMC: 2021 Oct 13.
Published in final edited form as: Mol Psychiatry. 2020 Aug 4;26(7):3122–3133. doi: 10.1038/s41380-020-0855-9

Figure 3.

Figure 3.

TSPAN5 regulates kynurenine concentrations. (a) Western blot analysis of TSPAN5 knockout clones for HMC3 cells. (b) Lower kynurenine concentrations (F3,12 = 10.54, p = 0.013) were observed in TSPAN5 knockout cells. (c-d) TSPAN5 mRNA expression was down-regulated in response to EtOH (F2,21 = 5.8, p = 0.018) or acamprosate treatment (F2,21 = 14.87, p = 0.0011) of HMC3 cells. (e) In parallel, kynurenine concentrations displayed significant decreases in the presence of EtOH (F2,21 = 15.8, p = 0.0009) or acamprosate (F2,21 = 19.24, p = 0.0005) for 24 hours. *p ≤0.05, as compared to wildtype cells or no drug treatment. Three independent experiments were performed. All values are mean±SEM.