Table 2.

Crystallization

Method: Sitting drop vapor diffusion

Plate type: PS MRC crystallization plate

Temperature (K): 100 K

Protein concentration: 10 mg/mL

Buffer composition of protein solution: 50 HEPES (pH 7.5), 100 mM KCl, 1 mM TCEP, 5% glycerol, and 2 mM inhibitor

Composition of reservoir solution:

TSA = 0.2 M potassium acetate and 20% PEG 3350

SAHA-BPyne = 0.2 M potassium sodium tartrate tetrahydrate and 20% PEG 3350

4-iodo-SAHA = 0.2 M magnesium chloride hexahydrate and 20% PEG 3350

Volume and ratio of drop: 1:1 ratio protein to precipitant solution

Volume of reservoir: 80 μL