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. Author manuscript; available in PMC: 2020 Sep 25.
Published in final edited form as: J Immunol. 2020 Aug 28;205(7):1810–1818. doi: 10.4049/jimmunol.1901056

Figure 2: Increased TLR signaling in αv-deficient Tlr7.1 transgenic B cells:

Figure 2:

(A) Sorted transitional (T1), marginal zone and follicular B cells from Tlr7.tg control and αv-CD19.Tlr7 mice were cultured with 5μg/ml TLR7 ligand R848 for 0–120 min. Nuclear NF-κB (p65) localization was measured by western blot and LSD1 is shown as nuclear loading control. (B,C) Cytokine (B) and IgG2c (C) levels in supernatants of cultured B cell sub-populations after stimulation with R848 for 24 hrs (cytokines, B) or 4 days (IgG2c, C). Data shown are mean±SD of replicate cultures from one experiment. p-values <0.05 (Student’s t-test) for comparisons between control Tlr7.tg and αv-CD19.Tlr7 mice are shown. Similar results were seen in at least 3 independent experiments.