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. Author manuscript; available in PMC: 2020 Sep 25.
Published in final edited form as: Alcohol Clin Exp Res. 2020 Jul 9;44(8):1686–1699. doi: 10.1111/acer.14396

Figure 4.

Figure 4.

The decreased sensitivity to alcohol is not linked to increased alcohol-induced hyperactivity when Gprk2 is knocked down in EB neurons. All experimental (cyan in a&b) and control groups were tested at the same time but presented for each RNAi line separately in a and b. Disruption of Gprk2 expression using two independent lines (GL00233 and 101463) with EB specific driver (5.30) showed no significant difference in 4-min peak activity (cyan in c) but decreased sensitivity to alcohol (cyan in d) compared to controls (black and gray in c&d). gprk2KO flies were used as positive controls (magenta, n=7). n=11 unless specified. Mean +/− SEM. ns, p>0.05, **p<0.01, ***p<0.001 according to the one-way ANOVA with Bonferroni post hoc test after the data passed the Kolmogorov-Smirnov normality test.