Figure 7.

Expression of Gprk2 in ellipsoid body neurons does not rescue the fragmented sleep patterns in Gprk2 knockout flies. Canton S (black), gprk2^KO rescue flies with two different Gal4 drivers (5.30 Rescue and ruslan Rescue, cyan), gprk2^KO control flies with either the Gal4 drivers or UAS transgene only (5.30 KO, ruslan KO, UAS-Gprk2 KO, gray) showed similar levels of locomotive activity (a) but decreased sleep time, specifically during night (b, c). The sleep time (c), bout number (d) and duration (e) were measured and compared between Rescue or gprk2^KO mutant control flies and the wildtype control (Canton S). For respective total, day and night sleep time, F_5,506 = 19.08, 5.779, 41.33. For the respective total, day and night bout number, H₅ = 184.3, 83.49, 200. For the respective total, day and night bout duration, H₅ = 164.4, 76.55, 196.4. One-way ANOVA test followed by Bonferroni posthoc (c) or Kruskal-Wallis tests followed by Dunn multiple comparison posthoc (d, e) were used in the statistical analysis. Means are indicated by + in each box. n=77-88 male flies for each genotype. ns, p>0.05, *p<0.05, ***p<0.001.