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. Author manuscript; available in PMC: 2020 Sep 8.
Published in final edited form as: Cell Mol Life Sci. 2015 Dec 26;73(9):1927–1937. doi: 10.1007/s00018-015-2118-5

Fig. 4.

Fig. 4

Mutation-induced alterations of ABCG2-mediated drug-efflux activity. HEK293 cells expressing wild-type ABCG2, the different C2-sequence mutants or the R482G gain-of-function mutant were incubated with 20 μM mitoxantrone with (blue line) or without (red line) 10 μM fumitremorgin C (FTC) as a specific inhibitor, to generate the histograms (a). The efflux was quantified by difference between the two histograms either without further corrections (b) or by taking into account the exact plasma membrane ABCG2 content determined in Fig. 3b (c)