Thrombus composition was assessed in thrombi from F12−/− and F11−/− mice formed using the IVC stasis model of thrombosis by western blotting of total soluble protein lysates and compared to respective wild-type littermate controls. Densitometric analysis of immunoblots for (A,H) fibrin(ogen), (B,I) CD41, (C, J) Ly6G, (D,K) β actin, (E,L) citrullinated histone H3 and (F,M) total histone H3 for F12−/−, F11−/− mice and wildtype littermate controls. (G,N) The ratio of H3Cit to total H3 was also calculated. *P<0.01 Mann-Whitney U test. Data represented as individual values plus the median.