Skip to main content
. Author manuscript; available in PMC: 2020 Nov 24.
Published in final edited form as: Cell Stem Cell. 2020 Sep 28;27(5):765–783.e14. doi: 10.1016/j.stem.2020.09.001

Figure 2: PRS locus enhancer clusters EC1.45 and EC1.25 are active in hCNCCs and during mouse craniofacial development.

Figure 2:

(A) ChIP-seq and ATAC-seq for PRS locus putative enhancer clusters EC1.45 (p300 peaks P1 and P2), EC1.35 (S1 and S2) and EC1.25 (S3, S4, S5 and S6).

(B) Luciferase reporter assays for EC1.45, EC1.35 (S1-S2) and EC1.25 (S3-S4-S5-S6) in hCNCC (left) and hESC (right).

(C) Schematic outlining craniofacial domains at E9.5 and E11.5. BA1–2, branchial arch 1–2; FNP, frontonasal prominence; LNP, lateral nasal process; MdP, mandibular process; MNP, medial nasal process; MxP, maxillary process.

(D) In situ hybridization (ISH) for Sox9 at E9.5 and E11.5.

(E) Mouse lacZ reporter assay for EC1.45, EC1.35 (S1-S2) and EC1.25 (S3, S4, S5, S6 tested individually) at E9.5 and E11.5.

(F) HREM for EC1.45 LacZ reporter embryo at E11.5 (frontal view, upper; parasagittal section, lower). White arrow, activity in MdP.

See also Figure S3 and Tables S1–2.