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. Author manuscript; available in PMC: 2020 Oct 9.
Published in final edited form as: STAR Protoc. 2020 Jun 27;1(2):100056. doi: 10.1016/j.xpro.2020.100056

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Biological Samples
Human tumor samples This study N/A
Chemicals, Peptides, and Recombinant Proteins
MammoCult™ Human Medium Kit Stemcell Technologies Ca#05620
Hydrocortisone Stock Solution Stemcell Technologies Ca#07925
Heparin Solution Stemcell Technologies Ca#07980
Matrigel Basement Membrane Matrix Corning Ca#354234
Collagenase type IV Thermo Fisher Ca#17-104-019
Dispase Life Technologies Ca#17105-041
Histogel Thermo Fisher Ca#R904012
Ammonium Chloride Solution Stem Cell Technology Ca#07850
Hanks’ Balanced Salt Solution (HBSS) Stem Cell Technology Ca#37150
Cryostor®CS10 Sigma Aldrich Ca#C2874
Recovery™ Cell Culture Freezing Medium Thermo Fisher Ca#12648010
PBS Thermo Fisher Ca#14190-144
10% Neutral Buffered Formalin VWR Ca#89370-094
Critical Commercial Assays
CellTiter-Glo 3D Promega Ca#G968B
AO/PI Staining Solution Nexcelom Ca#S2-0106-5mL
Software and Algorithms
Celigo S Software Nexcelom N/A
Other
Corning 96-well assay plate Corning Ca#CLS3610-48EA
Masterblock plate Greiner bio-one Ca#786261
MicroAmp Optical Adhesive Film Thermo Fisher Ca#4311971
Tissue Path Microcassette 6-Compartiment Thermo Fisher Ca#15182705C
Millipore Steriflip 50ml Thermo Fisher Ca#SE1M179M6
Cellometer Auto 2000 Cell Viability Counter Nexcelom N/A
Cellometer Disposable Imaging Chambers Nexcelom Ca#CHT4-SD100-002
Celigo S Image Cytometer Nexcelom Ca#200-BFFL-S
epMotion 96 Eppendorf Ca#5069000004
epT.I.P.S.® Motion as Reload System 50ul Eppendorf Ca#0030014421
epT.I.P.S.® Motion as Reload System 300ul Eppendorf Ca#0030014464
10μl Biotix™ uTIP™ Low-Retention Filter Pipette Tips Biotix Ca#M-0011-9FC
20μl Biotix™ uTIP™ Low-Retention Filter Pipette Tips Biotix Ca# M-0020-9FC
100μl Biotix™ uTIP™ Low-Retention Filter Pipette Tips Biotix Ca#M-0100-9FC
1000μl Biotix™ uTIP™ Low-Retention Filter Pipette Tips Biotix Ca#M-1000-9FC