KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Phospho-Histone H3 (Ser10) - PE conjugated (Clone 11D8) | Biolegend | Catalog # 650807 |
| Experimental Models: Organisms/Strains | ||
| Mouse (C57BL/6J) | Jackson Lab | # 000664 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DMEM/F12 | Thermo Fisher Scientific - Gibco | Catalog # 11330032 |
| DPBS, no calcium, no magnesium | Thermo Fisher Scientific - Gibco | Catalog # 14190144 |
| BSA | Millipore Sigma | Catalog # A2153–100G |
| N2 Supplement (100 X) | Thermo Fisher Scientific - Gibco | Catalog # 17502048 |
| B27 Supplement (50 X) | Thermo Fisher Scientific - Gibco | Catalog # 17504044 |
| Anti-Anti (100X) | Corning | Catalog # 30–004-CI |
| Normocin | InvivoGen | Catalog # ant-nr-1 |
| MACS BSA Stock Solution | Miltenyi Biotec | Catalog # 130-091-376 |
| autoMACS Rinsing Solution | Miltenyi Biotec | Catalog # 130-091-222 |
| mouse CD45 MicroBeads | Miltenyi Biotec | Catalog # 130-052-301 |
| EGF | PeproTech | Catalog # AF-100–15 |
| FGF | PeproTech | Catalog # 100–18B |
| In vivo jetPEI (includes 10% glucose solution) | Polyplus | Catalog # 201–10G |
| D-Luciferin Potassium Salt | Goldbio | Catalog # LUCK −3G |
| Red Blood Cell (RBC) lysis buffer (10X) | Biolegend | Catalog # 420301 |
| Fetal Bovine Serum (FBS) USDA Pure Premium | Peak Serum | Catalog # PS-FB3 |
| Critical Commercial Assays | ||
| Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit | Thermo Fisher Scientific Invitrogen | Catalog # C10636 |
| Recombinant DNA | ||
| pT2/SB100x-Luc | Addgene | Catalog # 20207 |
| pT/CAGGS-NRASV12 | Addgene | Catalog # 20205 |
| pT2/shP53 | Addgene | Catalog # 124261 |
| pKT-IDH1(R132H)-Katushka | Addgene | Catalog # 124257 |
| Software and Algorithms | ||
| FlowJo_V10 | Becton Dickinson | N/A |
| Other | ||
| LS Columns | Miltenyi Biotec | Catalog # 130-042-401 |
| QuadroMACS Separator | Miltenyi Biotec | Catalog # 130-090-976 |
| MACS MultiStand | Miltenyi Biotec | Catalog # 130-042-303 |
| Pre-Separation Filters | Miltenyi Biotec | Catalog # 130-041-407 |
| 10 μl syringe | Hamilton | 700 Series |
| 30 G hypodermic needle | Hamilton | Catalog # 7803–07. Specifications: 30GA RN 6PK 1.25” 15° |
| Cell strainers | Alkali Scientific Incorporated | Catalog # MT4070 |
| BD FACSAria™ III Flowcytometer | Becton, Dickinson & Company | N/A |
| Just for Mouse Stereotaxic Instrument | Stoelting | Catalog # 51730 |
| Quintessential Stereotaxic Injector (QSI) | Stoelting | Catalog # 53311 |
| IVIS Spectrum In Vivo Imaging System | Perkin Elmer | Catalog # 124262 |
| Allegra 6R Refrigerated Benchtop Centrifuge | Beckman Coulter | N/A |
| Small Animal Heat Lamp | Morganville Scientific | Catalog # HL0100 |
| Stereomicroscope System SZX7 (equipped with fluorescent lamp) | Olympus | N/A |
| 50 mL Olympus Polystyrene Tubes | Genesee Scientific Corporation | Catalog # 21–108 |
| 15 mL Olympus Polystyrene Tubes | Genesee Scientific Corporation | Catalog # 21–103 |
| CellPro™ Flow Cytometry Tubes without cap, sterile, 12×75mm | Alkali Scientific Incorporated | Catalog # CT6414 |
| Covidien Monoject 1 mL tuberculin Syringes | Fisher Scientific | Catalog # 22-257-154 |
| Friedman Bone Rongeurs | Stoelting | Catalog # 52160P |
| Micro Dissecting Scissors, 4.25 inch, Straight, 24mm, Sharp | Stoelting | Catalog # 52132–22P |
| Brown-Adson Forceps, 4.7in, Side Teeth | Stoelting | Catalog # 52104–31P |
| BD Precision Glide Hypodermic Needles 26G | Fisher Scientific | Catalog # 14-826-15 |