Skip to main content
. Author manuscript; available in PMC: 2021 Jun 10.
Published in final edited form as: Curr Res Toxicol. 2020 Sep 24;1:111–115. doi: 10.1016/j.crtox.2020.09.003

Figure 2: Effects of KIF and AUY-922 on P53 regulation.

Figure 2:

Western Bot analysis of (A) P53 and β-actin, (B) MDM2 and β-actin expression after 24 hours treatment of BPAEC with either KIF (5 μM) or vehicle (0.1% DMSO); and post-treatment with AUY-922 (2 μM) or vehicle (0.1% DMSO) for 16 hours. The blots shown are representative of 3 independent experiments. The signal intensity of P53 and MDM2 was analyzed by densitometry, and the protein levels were normalized to β-actin. *P < 0.05, **P<0.01 vs vehicle. Means ± SEM. Western Bot analysis of (C) P53 and β-actin, (D) MDM2 and β-actin expression after 24 hours treatment of HuLEC-5a with either KIF (5 μM) or vehicle (0.1% DMSO) and post-treatment with AUY-922 (2 μM) or vehicle (0.1% DMSO) for 16 hours. The blots shown are representative of 3 independent experiments. Signal intensity of P53 and MDM2 was analyzed by densitometry. Protein levels were normalized to β-actin. *P < 0.05, ***<0.001 vs vehicle. Means ± SEM.