Figure 2.

The PltL-PltF crystal structure. A) Adenylation and thiolation of L-Pro by PltF. B) Trapping of the PltL-PltF complex with Pro-AVSN (orange star). C) 2.15 Å resolution crystal structure of the PltL-PltF complex. PltL (pink) is trapped to PltF (purple) via Pro-AVSN (orange). PPant is shown in green. D) Close-up of the PltL-PltF interface. The left panel displays hydrogen bonding interactions. The right panel, which is rotated 180° from the left, displays hydrophobic interactions. The CSPs (cyan) from a recent NMR titration of PltL with PltF are mapped onto the bound PltL.