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Visualization of the PltL-PltF binding event. A) Linking of the solution NMR structure of holo-PltL (gray, PDB ID 2N5H), the CSPs (cyan) from the NMR titration, and the PltL-PltF (pink, purple) complex. B) Superposition of the bound (pink, cyan) and unbound (gray) PltL with PltF Ile454 (purple) and C) Phe231 (purple). Pro-AVSN was omitted for clarity. D) PltF mutant aminoacylation assay with PltL.
