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. Author manuscript; available in PMC: 2021 Jul 31.
Published in final edited form as: J Med Genet. 2020 Aug 20;58(7):484–494. doi: 10.1136/jmedgenet-2020-106987

Figure 4.

Figure 4

Wildtype and mutant CIC regulated the transcription of FOLR1 in stably transfected HeLa cells. (A) HeLa cells were transduced with lentiviral particles coding for GFP, GFP-CIC wildtype or GFP-CIC p.R353X. Representative western blots of endogenous FOLR1 (FRα), PCFT and RFC1 and overexpressed GFP-CIC. β-actin was used as a loading control. (B) Protein quantification of FOLR1, PCFT and RFC1 relative to GFP transfected HeLa cells (normalised against β-actin, n=3). (C) Quantification of FOLR1, PCFT and RFC1 mRNA levels in HeLa cells, stably transfected with GFP, GFP-CIC WT or GFP-CIC p.R353X. Expression was normalised against GAPDH (n=3). (D) Caco-2 cells were transduced with lentiviral particles coding for GFP, GFP-CIC wildtype or GFP-CIC p.R353X. Representative western blots of CIC and endogenous FOLR1 expression. B-actin was used as loading control. (E) Protein quantification of FOLR1 relative to GFP transfected Caco-2. (F) Protein localisation of wildtype and mutant type GFP-CIC in Caco-2 cells. Bars, 10 μm.