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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Neurosci. 2008 Jan 30;28(5):1109–1117. doi: 10.1523/JNEUROSCI.4853-07.2008

Figure 3. Mutation of −954 E-box in the wild-type −1696 α1T promoter prevents transgene induction in proliferating Müller glia following retina injury.

Figure 3

(a) Schematic representation of α1T promoter:GFP constructs. The wild-type −1696α1T:GFP transgene (−1696) and the 2 bp mutation created in the E-box at position −954 in the −1696 construct (TG-954CA) are indicated. (b) Three independent lines of transgenic fish received retinal injuries on day 0 and were given a 4 hr pulse of BrdU at 4 days post injury. Note that none of the lines harboring the 2 bp mutation induced transgene GFP expression in BrdU-labelled Müller glia (arrowheads). See Fig. 1 for comparison to the wild-type −1696α1T:GFP transgene response. The arrows in the lowest right GFP panel show transgene induction in retinal ganglion cells whose axons were presumably lesioned when we injured the retina in this fish.