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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Neurosci. 2008 Jan 30;28(5):1109–1117. doi: 10.1523/JNEUROSCI.4853-07.2008

Figure 5. Ascl1a regulates the α1T promoter through the −954 E-box.

Figure 5

(a) Luciferase reporter vectors with either a minimal β-globin promoter alone (light grey bars), or three copies of the −954 E-box (dark grey bars) or the mutant E-box from probe 4-4 (see Fig. 2b) (black bars) were transfected in combination with ascl1a into HEK293T cells. Ascl1a transactivates the reporter when a functional E-box is present, but not when the E-box is mutated. (b) Full-length α1T constructs harboring either the wild-type (−1696) or mutant E-box promoters were transfected into HEK293T cells with or without ascl1a. Ascl1a induces reporter expression when the E-box is intact, but not when the E-box is mutated. Error bars indicate standard error of the mean for three replicates.