Skip to main content
. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Neurosci. 2008 Jan 30;28(5):1109–1117. doi: 10.1523/JNEUROSCI.4853-07.2008

Figure 6. Ascl1a is required for transgene expression in vivo.

Figure 6

−1016 transgenic fish received retinal injuries and morpholino treatment on day 0. Eyes were harvested on day 4. (a) Control morpholino treatment has no effect on transgene expression in Müller glia (arrows) and there are many GFP+ Müller glia (arrowheads). Morpholinos targeting ascl1a (ascl1a and ascl1a5′UTR panels) prevent transgene expression in treated cells (arrows), and cause reduced transgene expression in general, although Müller glia that did not receive ascl1a morpholinos are able to express GFP (arrowheads). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.