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. Author manuscript; available in PMC: 2021 Feb 4.
Published in final edited form as: FASEB J. 2020 Nov 27;35(2):e21227. doi: 10.1096/fj.202002100R

Figure 6.

Figure 6.

Changes in TH+ cells in key regions of the hypothalamus important for feeding behavior. A. Western blot analysis of TH and SDH (represented by SDHB) in hypothalamic preoptic tissue for the indicated 4 representative animals. Western blot signal quantitation statistics were analyzed using a Student’s t-test with bars indicating mean ± sd. B. Fluorescent immunohistochemical staining analysis of the PVN monitoring TH (green), SDHB (red) and DAPI (blue) Arrows indicate example cells showing contrasting features. Scale bar: 50 μm.