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Gene expression changes due to genome editing of enhancers. Genomic regions corresponding to enhancer regions 1 and 4 were targeted for deletion in SW948 cells using CRISPR/Cas9 technology. Pools of transfected cells were analyzed using qPCR and Taqman gene expression assays for GREM1, FMN1 and GUSB (control). Targeting of both region 1 and 4 enhancers resulted in a decrease in GREM1 expression levels, while FMN1 expression levels did not change significantly.
