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. Author manuscript; available in PMC: 2021 Feb 23.
Published in final edited form as: Bio Protoc. 2020 Sep 20;10(18):e3761. doi: 10.21769/bioprotoc.3761

Figure 2. Inhibition of the cell surface transport of α2B-AR by mutating residues Y12/S13, L48, F436 and I443/L444.

Figure 2.

Wild-type and mutated α2B-AR were transiently expressed in HEK293 cells and their surface expression was measured by radioligand binding of intact live cells using [3H]-RX821002.