Skip to main content
. Author manuscript; available in PMC: 2021 Feb 23.
Published in final edited form as: Bio Protoc. 2020 Sep 20;10(18):e3761. doi: 10.21769/bioprotoc.3761

Table 1.

Measurement of the surface expression of GPCRs by intact cell ligand binding assays

Receptors Radioligands References
α1-AR (α1A- and α1B-AR) [3H]-Prazosin (Phentolamine) Duvernay, 2009a and 2009b; Zhang et al., 2011; Li et al., 2017
α2-AR (α2A-, α2B- and α2C-AR) [3H]-RX821002 (Rauwolscine) Dong and Wu, 2007; Dong et al., 2008; Duvernay 2009a and 2009b; Dong et al., 2010a and 2010b; Zhang et al., 2011; Filipeanu et al., 2015; Li et al., 2017; Wei et al., 2019
β-AR
(β1- and β2-AR)		 [3H]-CGP12177
(Alprenolol)		 Dong and Wu, 2007; Dong et al., 2008; Duvernay 2009a and 2009b;
Dong et al., 2010a and 2010b; Zhang et al., 2011; Filipeanu et al., 2015; Li et al., 2017; Wei et al., 2019
AT1R and AT2R [125I]-Ang II
(Ang II)		 Filipeanu et al., 2004; Dong and Wu, 2007; Dong et al., 2008; Duvernay et al., 2009a and 2009b; Dong et al., 2010b; Zhang et al., 2011
M3-MR [3H]-NMS
(Atropine)		 Dong et al., 2010b

Drugs indicated in ( ) are used for measurement of non-specific radioligand binding.