Fig. 1.

Antitrypanosomal drugs: effects on cell cycle and DNA synthesis. (A) Model depicts the enzymes of the trypanothione pathway: γ-glutamylcysteine synthetase (GSH1), glutathione synthetase (GSH2), ornithine decarboxylase (ODC), spermidine synthetase (SpS), trypanothione synthetase (TryS), trypanothione reductase (TR), tryparedoxin (TXN), glutathione-peroxidase-type enzymes (Px), 2-cys-peroxidases (Prx) and ribonucleotide reductase (RR). Antitrypanosomal drugs and their association with the pathway are also depicted. (B) Sample gating controls from flow cytometry analysis of cell cycle by propidium iodide (top panel) and DNA synthesis analysis by BrdU incorporation assay (bottom panel). (C) Effects of drugs on cell cycle after 24 h of treatment at the indicated drug concentrations. (D) Effects of DNA synthesis after 24 h of drug treatment followed by 1 h of BrdU incorporation. Flow cytometry data are presented as a single replicate representative of at least three biological replicates.