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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Microbiology (Reading). 2009 Dec 24;156(Pt 4):1120–1133. doi: 10.1099/mic.0.032896-0

Figure 5.

Figure 5

Flagella-based motility is required for optimal invasion of epithelial cells. (a) Bacterial motility was assessed by fluorescence microscopy using S. Typhimurium constitutively expressing GFP. Shown are means± SD from three independent experiments. P<0.05, Student's t-test. (b) Comparison of the role of flagellar-based motility in invasion. HeLa cells were infected with aer-LL or μaer-ST S. Typhimurium either with (grey bars) or without (black bars) centrifugation. Intracellular bacteria (1 h p.i.) were enumerated by plating. Strains were wild type S. Typhimurium (WT), fliC::Tn10 fljB::Mud-Cm mutant (fliC/fljB) and a ΔSPI1::kan mutant (SPI1). Shown are means ± SD from at least three independent experiments. *= P<0.05 Student's t-test (compared to WT without centrifugation).