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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Microbiology (Reading). 2009 Dec 24;156(Pt 4):1120–1133. doi: 10.1099/mic.0.032896-0

Figure 6.

Figure 6

Intracellular replication is affected by pre-invasion growth conditions. (a) Infected HeLa cells were lysed for enumeration of intracellular S. Typhimurium or (b) fixed and processed for immunofluorescence using anti-LPS and anti-LAMP1 antibodies. To obtain comparable invasion cells were infected with an m.o.i. of ~50-60 for aer-LL and ~150-180 for μaer-ST. The number of intracellular aer-LL (black symbols) or μaer-ST (white symbols) S. Typhimurium with coincident LAMP1 staining cell was scored visually by fluorescence microscopy. Shown are means ± SD from three independent experiments. *= P<0.001, Bonferroni's post-hoc test.