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. Author manuscript; available in PMC: 2021 Jul 2.
Published in final edited form as: Chembiochem. 2021 Jan 27;22(8):1400–1404. doi: 10.1002/cbic.202000854

Figure 2. Oligodeoxyribonucleotide 5’ FAM modification is necessary and sufficient for efficient radical biotinylation catalyzed by HRP in the presence of H2O2 and biotin tyramide.

Figure 2.

Biotinylation of the indicated DNA oligonucleotides is detected by a streptavidin-dependent gel mobility shift under native conditions. Statistics are calculated from independent experiments performed in triplicate; S.D. is standard deviation.