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. Author manuscript; available in PMC: 2021 Jul 2.
Published in final edited form as: Chembiochem. 2021 Jan 27;22(8):1400–1404. doi: 10.1002/cbic.202000854

Figure 4. Radical biotinylation of FAM but not TAMRA.

Figure 4.

A. Structures of FAM and TAMRA highlighting phenolic group of FAM (red) vs. amino groups of TAMRA (blue). R: oligonucleotide. B. Absence of detectable radical biotinylation under native gel conditions of 5’ TAMRA-modified LJM-6223 relative to 5’ FAM-modified LJM-6132. Statistics are calculated from independent experiments performed in triplicate; S.D. is standard deviation.