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. Author manuscript; available in PMC: 2021 Apr 27.
Published in final edited form as: JACC CardioOncol. 2021 Mar 16;3(1):113–130. doi: 10.1016/j.jaccao.2020.12.006

Figure 2. SS rats demonstrate myocardial vacuolization and necrosis, with a marginally significant decrease of microvascular density, 10-weeks after 24Gy localized cardiac RT.

Figure 2.

Adult female rats were administered 24Gy of localized cardiac RT or sham treatment, and at 10-weeks post-treatment their hearts were harvested. H&E stained sections demonstrated healthy LV myocardial tissue in sham-treated hearts (a-b), while RT-treated hearts had notable necrosis and vacuolization (c-d), which was increased in the LV lateral wall (d) when compared to the interventricular septum (c). (e-g) Fixed heart sections were stained for cardiomyocytes (caveolin-3) and blood vessels (Sh2b3). The number of blood vessels per cardiomyocyte was calculated, with representative images from sham-treated (e) and RT-treated (f) hearts. This demonstrated a marginally significant decrease of capillary density after RT (g). N=3/condition. Values are the mean±SEM.