Platelet activation defects and reduced thrombus formation lead to bleeding complications 3 days after PHx. (A) Expression of indicated glycoproteins on the surface of platelets measured via MFI in flow cytometric analysis (n= 13 sham, 18 PHx). (B) Externalization of P-selectin on the platelet surface with indicated agonists (n= 9 sham, 12 PHx). (C) Externalization of the β3 Integrin subunit upon platelet stimulation (n= 8 sham, 9 PHx). (D) Activation of αIIbβ3 integrin on the platelet surface with indicated agonists (n= 9 sham, 12 PHx). (E) Thrombus formation on a collagen matrix under arterial shear rates with representative pictures (1000s−1 and 1700s−1 n= 3 sham, 4 PHx). (F) In vivo analysis of hemostatic dysfunction via tail bleeding time (n= 9 sham, 8 PHx). Depicted are mean values + s.e.m.; *P<0.05, **P<0.01 using paired students t-test.