FIG. 5.
Elevated megakaryopoiesis is responsible for moderate splenomegaly after PHx. (A) Calculated spleen weight ratio (n = 1d: 10 sham, 14 PHx; 3d: 9 sham, 9 PHx; 7d: 8 sham, 10 PHx, 14d: 5 sham, 5 PHx) with (B) representative images of spleen tissue 7 days after operation. (C) Platelet sequestration in spleen sections of sham and PHx operated mice. A platelet specific marker (GPIX) was combined with DAPI staining to distinguish between red and withe pulp of the spleen. Total GPIX positive fluorescence area [IFGPIX/ μm2] of the red pulp of the spleen sections was determined with (D) representative IF staining of splenic sequestration (n= 4). (E) Counted megakaryocytes in paraffin embedded spleen tissue (n = 5 sham, 5 PHx) and (F) bone marrow of the femur (n= 4 sham, 4 PHx). (G) Representative images from the spleen tissue and (H) the bone marrow tissue of the HE-staining. Arrowheads indicate megakaryocytes. Depicted are mean values + s.e.m.; *P<0.05, **P<0.01 using ordinary Two-Way ANOVA with Bonferroni’s post-hoc test.