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. Author manuscript; available in PMC: 2021 May 26.
Published in final edited form as: J Parkinsons Dis. 2021;11(2):585–603. doi: 10.3233/JPD-202351

Figure 3. Adoptive transfer of B cells alone did not alter phosphorylated α-syn pathology in immunocompromised mice.

Figure 3.

Figure 3.

a. Timeline of experiment. b. Flow cytometric analysis of mouse spleen and blood following adoptive transfer demonstrated that wildtype mice contain populations of T and B cells that are CD3 and CD19 positive. NSG mice do not have T and B cell populations. Following adoptive transfer of B cells, NSG mice contained CD45.2+ CD19+ B cells. Representative plots are shown for each treatment condition. c. Phosphorylated α-syn was detected in the ipsilateral striatum, substantia nigra and frontal cortex. d. Densitometry of 3–9 mice per group to determine the area covered in phosphorylated α-syn levels in the ipsilateral striatum, substantia nigra and frontal cortex. Wildtype Saline, n = 5; NSG Saline n = 3, wildtype PFFs, n = 3; NSG PFFs, n = 3; NSG PFF B n = 12). The error bars represent S.E.M. Statistical analyses were performed by Kruskal-Wallis test * p < 0.01. Scale bar: 100 μm. Schematic created with BioRender.com