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. Author manuscript; available in PMC: 2021 Sep 28.
Published in final edited form as: Cell Microbiol. 2021 Jun 9;23(9):e13350. doi: 10.1111/cmi.13350

Figure 3. Colonization of mice organs by B. burgdorferi as detected by light emission due to the presence of live bioluminescent N40 strain at three weeks of infection.

Figure 3.

(a) Representative real-time images of five C3H/HeJ (HeJ) and C3H/HeN (HeN) mice infected with bioluminescent N40 strain using IVIS-200 displaying bioluminescence in the head and joints regions. Bioluminescence radiance in the whole body of each mouse (as marked in one mouse by a red rectangle) was also measured. (b) Bioluminescence detection in co-infected C3H/HeJ and C3H/HeN mice. (c) Live imaging of a set of five uninfected mice at the same time point. (d) Average bioluminescence radiance from five uninfected mice was deducted from the radiance obtained for each infected mouse and net radiance data for each mouse is shown. Horizontal lines indicate the comparison of net mean radiance in each group of infected mice. Difference between average bioluminescence radiance in N40-infected C3H/HeJ and co-infected C3H/HeN mice was not statistically significant (NS). Statistical analysis was conducted using a two-tailed unpaired student t tests for unequal variance to determine significant difference between the paired groups (***p<0.001, ****p<0.0001).