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. Author manuscript; available in PMC: 2022 Jan 15.
Published in final edited form as: Mol Psychiatry. 2020 Jul 10;26(10):5766–5788. doi: 10.1038/s41380-020-0806-5

Fig. 2. FRET-based assay for BACE2 cleavage.

Fig. 2

A newly custom designed FRET reagent (spanning the Aβ34 site) was digested at pH=3.5 by the human BACE2 in presence or absence of the stated inhibitors for 2 h. Enzyme activity was defined by measuring the fluorescence increase before and after the incubation. Blank-subtracted fluorescence units were normalized to the control digest and a one-way ANOVA was performed. P values were calculated with a post hoc Bonferroni multiple comparison (only pairs relative to the untreated control simultaneously compared). Error bars: standard error. n = 3 replicates per inhibitor per concentration.