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. Author manuscript; available in PMC: 2022 Jan 15.
Published in final edited form as: Mol Psychiatry. 2020 Jul 10;26(10):5766–5788. doi: 10.1038/s41380-020-0806-5

Fig. 9. Tau pathology, staining with Gallyas, and with three different antibodies (hyperphosphorylated, conformationally altered, and filamentous Tau) in QM-DupAPP (100DIV) and QM-DS2 (100DIV) organoids.

Fig. 9

a Scan of whole QM-DS2 organoid section shows two hyperphosphorylated Tau foci (neuritic plaque-like structures). Scale bar: 500 μm. b Zoom in on the same foci. Scale bar: 100 μm. c, d AT8-positive neurites in the pathological structure number 1 in two individual z-slices. Scale bar: 20 μm. e, f AT8-positive neurites in the pathological structure number 2 in two individual z-slices. Scale bar: 20 μm. g–i AT8-positive neurites in further three pathological foci found at a different depth, from the same organoid (not shown at lower magnification). Scale bar: 20 μm. j–m AT100 (filamentous Tau) positive neurons in the cortical layer of QM-DS2 organoid, partly showing “ballooned neuron” pathology. Scale bar: 5 μm. n TG3 (conformationally altered Tau) positive cells in the cortical layer of QM-DS2 organoid. Scale bar: 50 μm. o Scan of whole QM-DS2 organoid section stained with Gallyas. Scale bar: 500 μm. p–r zoom in on the parts of the same organoid shows strongly Gallyas-positive individual neurons. Scale bar: 50 μm p 20 μm q, r. s Scan of whole QM-DupAPP organoid section stained with Gallyas. Scale bar: 500 μm. t Zoom in on the same organoid shows equally strong individual neurons as in QM-DS2 organoid. Scale bar: 50 μm.