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. Author manuscript; available in PMC: 2022 Apr 20.
Published in final edited form as: Methods. 2021 May 3;201:49–64. doi: 10.1016/j.ymeth.2021.04.025

Figure 4. Dynamic range of the SIV DNA ddPCR assay on DNA derived from a tissue sample.

Figure 4.

DNA (in quantities from 0.11 million cell equivalent to 4 million cell equivalent genomic DNA) extracted from the ovary tissue of an SIV-infected, cART suppressed Rhesus macaque, was analyzed for SIV DNA with the SIV ddPCR assay on the Raindance platform. The DNA input upper limit tested in each reaction was 4 million cell equivalent genomic DNA, as DNA input higher than this amount was shown to cause reaction inhibition [19]. The numeric values for measured DNA viral loads were: 0.11 million cell equivalent input, n=3, 4±1; 0.33 million cell equivalent input, n=3, 19±2; 1 million cell equivalent input, n=3, 62±5; 2 million cell equivalent input, n=2, 118 (range 110–125); 2.5 million cell equivalent input, n=2, 160 (range 151–168); 3 million cell equivalent input, n=2, 188 (range 177–198); 4 million cell equivalent input, n=2, 256 (range 251–261). Note that for 2 to 4 million cell equivalent DNA input, each input was tested in duplicate due to total sample quantity limitation.