Component	Original	Final	μL per 15 μL reaction
MgCl2	25 mM	5 mM	3
dNTPs	25 mM	0.5 μM	0.3
DTT	100 mM	1 mM	0.15
SIVNestR01	100 μM	2 μM	0.3
PCR II w/ 0.2% Tween 20	10x	1x	1.5
RNaseOUT	40 U/μL	10 U	0.25
SSIV RT enzyme	200 U/μL	200 U	1
RNA sample or reference standard	Various	various	various
H2O			various