| Component | Original | Final | μL per 50 μL reaction |
|---|---|---|---|
| TaqMan genotyping master mix | 2x | 1x | 25 |
| Target assay forward primer (SGag forward) | 100 μM | 600 nM | 0.3 |
| Target assay reverse primer (SGag reverse) | 100 μM | 600 nM | 0.3 |
| Target assay probe (SGag ddPCR probe) | 10 μM | 200 nM | 1 |
| Reference assay forward primer (RCCR5 forward) | 100 μM | 200 nM | 0.1 |
| Reference assay reverse primer (RCCR5 reverse) | 100 μM | 200 nM | 0.1 |
| Reference assay probe (RCCR5 ddPCR probe) | 10 μM | 200 nM | 1 |
| Droplet stabilizing solution | 25x | 1x | 2 |
| DNA sample or reference standard | various | various | various |
| H2O | various |
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