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. Author manuscript; available in PMC: 2022 Apr 20.
Published in final edited form as: Methods. 2021 May 3;201:49–64. doi: 10.1016/j.ymeth.2021.04.025

Table 1.

Sequences of primers and probes used in the reverse transcription and ddPCR steps

Primer Sequence Note
SIVNestR01 GTTGGTCTACTTGTTTTTGGCATAGTTTC
SGag forward GTCTGCGTCAT(dP)TGGTGCATTC dP [50] denotes a nonstandard base
SGag reverse CACTAG(dK)TGTCTCTGCACTAT(dP)TGTTTTG dK [50] and dP denote nonstandard bases
RCCR5 forward CCAGAAGAGCTGCGACATCC
RCCR5 reverse GTTAAGGCTTTTACTCATCTCAGAAGCTAAC
Probe Sequence
SGag ddPCR probe 5’-FAM- CTT CYT CAG TRT GTT TCA CTT T -MGB
RCCR5 ddPCR probe 5’ VIC- TTC CCC TAC AAG AAA CT-MGB