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. Author manuscript; available in PMC: 2021 Oct 7.
Published in final edited form as: Plant Biotechnol J. 2021 Jun 22;19(10):2113–2125. doi: 10.1111/pbi.13643

Figure 2: Chloroplast derived Marker-free PG1-dextranse and mutanase enzyme activity.

Figure 2:

Enzyme extracted from Marker-free PG1-dextranse lyophilized leaf powder from harvested leaves and evaluated for the qualitative assay against 0.5% blue dextran on plate (A), quantitative enzyme assay against 1% dextran (B), release of enzyme in the plant crude extract with or without sonication (C), and enzyme stability evaluation of protein extracted in presence/absence of protease inhibitors (D). Marker-free mutanase enzyme activity (E). Enzyme activity calculated by measuring released reducing sugars and compared with the maltose standard. Assays were performed in triplicates and data represents mean and standard deviation. Statistical significance analyzed by t-test. Statistical significance was set at P<0.05 (*), p<0.01 (**) and P<0.001 (***). WT represents untransformed wild type plant (–ve control); NS represents Not Significant.