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. Author manuscript; available in PMC: 2021 Oct 7.
Published in final edited form as: Plant Biotechnol J. 2021 Jun 22;19(10):2113–2125. doi: 10.1111/pbi.13643

Figure 5: Viability of the fungal-bacterial mixed biofilm after sequential treatments with commercial Lipase and Dextranse/Mutanse combination.

Figure 5:

(A) Total Biofilm Inhibition (TBI) index of the treatments. TBI = Ifungal CFU × Ibacterial CFU × IDW, where Inhibition of bacterial/fungal CFU or ICFU = CFU treatmentCFU control × 100%, and Inhibition of Dry Weight or IDW = DW treatmentDW control × 100%. *, p<0.05; **, p<0.01 (1-way analysis of variance with Tukey’s multiple comparisons test). (B) Live/dead staining of the fungal-bacteria mixed biofilms. Live cells are depicted in green; Dead cells are depicted in magenta. C. albicans cell wall is depicted in cyan to indicate the location of fungal cells. The optimum activity units (U) were used for commercial purified lipase (1000 U/mL) and Dex/Mut (525/105 U/mL) in the experiments. Enzyme unit of lipase and dextranase/mutanase represent μmol of pNP and reducing sugar produced in 1 hour, respectively.