| Reagent | Amount |
|---|---|
|
| |
| Nuclease-free water | x μl (Total reaction volume 30 μl) |
| ATP (100 mM) | 1.5 μl |
| UTP (100 mM) | 1.5 μl |
| GTP (100 mM) | 1.5 μl |
| CTP (100 mM) | 1.5 μl |
| 10X T7 reaction Buffer | 3 μl |
| T7 RNA Polymerase Mix | 2 μl |
| Purified DNA Template | x μl (~ 600 ng) |
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