| Reagent | Amount |
|---|---|
|
| |
| Nuclease-free water | x μl |
| Purified custom 5’sgRNA (e.g.NBS1 sgRNA-B) | x μl (16 μg) |
| Purified custom 3’sgRNA (e.g. NBS1 sgRNA-C) | x μl (16 μg) |
| 10X reaction Buffer 3.1 | 20 μl |
| Cas9 Nuclease (20 μM) | 4 μl |
| Purified Genomic DNA | x μl (5 μg) |
|
| |
| Total reaction volume | 100 μl |
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