A) Representative images of WT and Ehhadh KO kidneys from male mice. B, C) Measurement of B) kidney weight (combined weight of both kidneys per animal) and C) kidney-to-body weight (BW) ratio in 4 to 9 month-old male WT (n=8), male Ehhadh KO (n=9), female WT (n=9), and female Ehhadh KO mice (n=12). D) Representative images of H&E and PAS staining of kidney sections from male WT and Ehhadh KO mice. Scale bars = 100 μm. E) Morphometric analysis of the cross-sectional tubule areas in WT (n=6) and Ehhadh KO (n=7) male mice F) Glomerular filtration rate (GFR) in WT (n=6) and Ehhadh KO (n=4) male mice. G) Plasma creatinine levels (μmol/L) in WT (n=5) and Ehhadh KO (n=7) male mice. H) Blood urea nitrogen (BUN) levels (mg/dL) in WT (n=7) and Ehhadh KO (n=8) mice male. Data are presented as mean ± SD with individual values plotted. ***P < 0.001, **P < 0.01, *P < 0.05, by two-way ANOVA (B and C), or unpaired t-test with Welch’s correction (E, F).