Skip to main content
. Author manuscript; available in PMC: 2021 Oct 13.
Published in final edited form as: Kidney360. 2021 Sep 30;2(9):1441–1454. doi: 10.34067/KID.0003772021

Figure 4. Metabolite profiling in Ehhadh KO male kidneys.

Figure 4.

A) Pathway enrichment analysis using significantly altered HMDB metabolites. Scatterplot represents unadj p values from integrated enrichment analysis and impact values from pathway topology analysis. The node color is based on the p values and the node radius represents the pathway impact values. The only significantly altered KEGG pathway using Fisher exact t-test (adj p < 0.05) was “Sphingolipid metabolism”. B) Glucosyl- and lactosyl-ceramides levels in WT (n=7) and Ehhadh KO (n=5) male mice. The scaled imputed data (Scaled Imp Data) represent the normalized raw area counts of each metabolite rescaled to set the median equal to 1. Any missing values are imputed with the minimum value. C) Immunoblots of SPTLC2 and the loading control citrate synthase in WT (n=3 per sex) and Ehhadh KO (n=4 per sex) mice, and the corresponding quantification. Data are presented as mean ± SD with individual values plotted (B, C). Statistical significance was tested using unpaired t test with Welch’s correction (B) or two-way ANOVA with “Genotype” and “Sex” as the two factors, followed by Tukey’s multiple comparisons test (C). *P < 0.05; **P < 0.01; ***P < 0.001.