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. Author manuscript; available in PMC: 2021 Oct 21.
Published in final edited form as: Mol Microbiol. 2021 Jul 12;116(3):877–889. doi: 10.1111/mmi.14777

FIGURE 6.

FIGURE 6

Expression of RecA, RumA and RumA’ in wild-type recA+ lexA+ cells after exposure to the SOS-inducing antibiotic, Ciprofloxacin.

Western blot analysis was performed on whole-cell protein extracts from RW520 (recA+ lexA+) harboring pRLH421 derivatives (pJM1355, pJM1356, pJM1359 or pJM1360), with various croSR391 or setRR391 wild-type, or deletion combinations as indicated. To induce the SOS response, cells were treated with 30 ng ml−1 Ciprofloxacin for various times, as indicated in the figure. Levels of RecA and RumA/ RumA’ were detected using affinity purified polyclonal rabbit antibodies to RecA and RumA proteins. The number reported for the level of RumA or RumA’ are relative to a cross-reacting band in the same track.