galK2(Oc) reversion papillation assay of R391, or R391 ΔcroS strains.
R391, or R391 ΔcroS were moved into MVG114 by conjugal transfer. Cells were plated onto MacConkey-galactose agar media and grown for 8 days at 37°C. Pictures of representative colonies from each strain were taken showing the appearance of Gal+ papillae indicating the level of mutagenesis occurring within the colonies. The MVG114/R391 ΔcroS colonies contain approximately 10 −50 times the number of revertant papillae, when compared to the MVG114/R391 colonies.