Skip to main content
. Author manuscript; available in PMC: 2021 Oct 21.
Published in final edited form as: Mol Microbiol. 2021 Jul 12;116(3):877–889. doi: 10.1111/mmi.14777

Table 3.

Oligonucleotides used in this study

Name Sequence Source

P1a CTCGCTTTTGTGTTTACGTAAAATGCTGACCATTATTTGCCCTTAATCCTAAATTAACCCTCACTAAAGGGCGG BioSpring
P2a CAGTTTAAACTGTAACATTCAATCTTGTAACAGTTATTATTGTAACAGGAGACGGTTAGCACGGAGTTCATTAGGGCTC Biospring
cp01b GCGATGCCATACCAATGAAGTCG BioSpring
cp02b CAACGTAACCAATCGGCAGTCG BioSpring
cp03b CCAGTGACTTCACGCCACTCC BioSpring
cp04b CAGGATCAACAACGATCACTGCC BioSpring
a:

Used in the generation of linear knock out cassette.

b:

Used in the amplification of the modified region and subsequent DNA sequencing