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. Author manuscript; available in PMC: 2021 Nov 4.
Published in final edited form as: Mucosal Immunol. 2021 Jul 21;14(6):1295–1305. doi: 10.1038/s41385-021-00430-6

Figure 4: CISH constrains proliferation and cytokine production in ILC2s.

Figure 4:

a. Gene set enrichment analysis of small intestinal ILC2s from WT or CISH KO mice.

b. Gene set enrichment analysis of lung ILC2s from WT or CISH KO mice. For (a-b), each column represents sequenced cells from an individual mouse. Color blocks indicate row normalized expression. n = 4 mice/group.

c. Number of live ILC2s after 1 week of culture in 10 ng/mL IL-2 and IL-7. **p<0.01 by unpaired t-test. for (c-f), n = 5 wells/group with each well derived from a single mouse.

d. Histogram (left) and graphical quantitation (right) of CellTrace Far Red dye dilution in cultured ILC2s. “fresh” indicates cells immediately after staining, without subsequent culture. ***p<0.001 by unpaired t-test.

e. Forward scatter (FSC) and side scatter (SSC) of cultured ILC2s after 1 week of culture. ***p<0.001 by unpaired t-test.

f. R5 MFI in cultured ILC2s. ***p<0.001 by unpaired t-test.

g. IL-5 from culture supernatant of cells cultured for 4d in IL-2 and IL-7. ****p<0.0001 by unpaired t-test. n =4 wells/group, derived from a split pooled collection of ILC2s from 8 mice/genotype.

h. IL-13 from culture supernatant of cells cultured for 4d. *p<0.05 by unpaired t-test. n=4 wells/group.